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Primers and probe designed to target the mcr-1 gene.
| Primer/Probe Name | Target Sequences | Sequence (5’–3’) | Tm (°C) | Product Length (bp) | Reference |
|---|---|---|---|---|---|
| qPCR | locus region 22541-22635 in the KP347227 | 116 | This study | ||
| M-F | CATCGCGGACAATCTCGG | 57.2 | |||
| M-R | AAATCAACACAGGCTTTAGCAC | 55.9 | |||
| M-Probe | FAM-AACAGCGTGGTGATCAGTAGCAT-BHQ | 61.4 | |||
| Standard-PCR | locus region 22447-22755 in the KP347227 | 305 | [16] | ||
| CLR5-F | CGGTCAGTCCGTTTGTTC | 56.1 | |||
| CLR5-R | CTTGGTCGGTCTGTAGGG | 56.3 |
