Figure 2.

Receptor-mediated uptake of glycolipid antigens and costimulatory properties remain unchanged in Atg5-DC CKO DCs. CD1D1-presentation of indicated iNKT cell agonists by primary splenic DCs was assessed using activation of the iNKT hybridoma A.407 as in Fig. 1B (A). Splenic DCs derived from either Atg5 floxed Ctrl or Atg5-DC CKO animals were stained for CD40 surface expression. Pooled data and SEM of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (B). Frequency of IL12B-producing splenic DCs upon CD40 ligation. Purified splenic DCs were either incubated with anti-CD40 overnight or left untreated. IL12B production was measured via intracellular cytokine staining and by ELISA. Results of 3 independent experiments are shown. Each experiment contained at least 3 animals per group. Statistics: 2-tailed unpaired Student t test (C). *** P≤ 0.001,** P≤ 0.01,* ≤ 0.05, ns P> 0.05. MFI, mean fluorescence intensity.