Table 5.

Reductive amination of bulky–bulky ketones and aldehyde employing AmDHs

Entry	No.	Enzyme	Enzyme concentration [µM]	Time [h]	Conversion substrate [%]	ee% (R)
1	20a	Ch1-AmDH	90	48	57	>99
2		Rs-PhAmDH	100	48	32	>99
3	21a	Rs-PhAmDH	130	48	>99	>99
4	22a	Rs-PhAmDH	100	48	71	>99
5	23a	Rs-PhAmDH	100	48	87	>99
6	24a	Rs-PhAmDH	50	24	>99	>99
7	25a	Rs-PhAmDH	100	48	96	n.a.

Reaction conditions: 0.5 mL, 50 mM substrate, 30–130 µM enzyme, ammonium formate buffer (1.005 M, pH 8.5), 14 µM Cb-FDH. n.a.: not applicable.