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. 2004 Dec 23;24(3):464–472. doi: 10.1038/sj.emboj.7600537

Figure 2.

Figure 2

TX100-solubilised rhomboids were tested for the cleavage of three model substrates based on membrane-anchored Drosophila EGF-type ligands. (A) Cleavage assay using Gurken-TMD, (B) Spitz-TMD and (C) Delta-TMD with TX100-solubilised B. subtilis YqgP (Yq), B. subtilis YdcA (Yd), E. coli GlpG, (Ec), P. aeruginosa rhomboid (Pa), P. stuartii AarA (Ps), A. aeolicus rhomboid (Aa), Drosophila melanogaster Rhomboid-1 (Dm) and human RHBDL2 (Hs); b, buffer only. (D) Recombinant expression and solubilisation of mutant rhomboids was analysed by Western blotting. After solubilisation with TX100, similar amounts of monomeric form and, to varying extents, putative multimeric species were observed.