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. 2005 Jan 20;24(3):580–588. doi: 10.1038/sj.emboj.7600540

Figure 3.

Figure 3

NAR1 is genetically linked to RIX19 and is required for nuclear export of ribosomes. (A) The rix19/nar sl mutant was transformed with pRS315 plasmid without insert or containing NAR1 or RIX19. Cells were grown on 5-FOA containing SDC plates for 5 days at 30°C. (B) The NAR1 shuffle strain was transformed with pRS315 plasmids containing either NAR1 or nar1-2. After plasmid shuffling on 5-FOA containing SDC plates, the cells were grown in serial dilutions on YPD plates at the indicated temperatures for 3 days. (C, D) nar1-2 strain was transformed with Rps2-GFP and Rpl25-GFP plasmids. Cells growing in liquid SDC medium at 30°C were observed in the fluorescence (C) and confocal (D) microscope. (E) Poly(A)+ RNA localization in nar1-2 cells, grown at 30°C, was analyzed by in situ hybridization.