Figure 6.

Rli1 is associated with pre-rRNA, rRNA and eIF3. (A) Immunoprecipitation of TAP-tagged Rli1 and Ded1 was performed in the presence of 150 mM NaCl using IgG-Sepharose. RNAs were extracted from the pellets obtained after precipitation (lanes IP) or from an amount of total extract corresponding to 1/30 of that used for the precipitation (lanes T). Co-precipitated RNAs were identified by Northern hybridization. (B) TAP purification of Rli1 was performed in the presence of 150 mM NaCl (lane 2) or 50 mM NaCl (lane 3). Lane 1 depicts a protein standard. Bands marked with asterisks are the commonly found contaminants in TAP purifications. (C) RLI1 and HCR1 are genetically linked. The hcr1Δ strain pretransformed with pRS315-RLI1 plasmid was crossed with RLI1-TAP strain. After subsequent tetrad analysis, two haploid strains from one tetrad carrying both mutations were selected and plated on FOA-containing media together with parental strains. (D) Ribosomal export is impaired in hcr1Δ cells. hcr1Δ strain was transformed with Rpl25-GFP or Rps2-GFP plasmids. The cells were grown in liquid SDC-leu medium at 30°C. Cells were examined in the fluorescence microscope.