Figure 1.

Self-renewal and multilineage differentiation of neurosphere cells. (A) Brain-derived cells from wt and eGFP/bcl2 double transgenic E 14.5 embryos form floating spheres (neurospheres) in culture. (B) To assess the ability of wt and eGFP/bcl2 neurosphere cells to self-renew, graded numbers of cells from single-cell suspensions of neurospheres were seeded into 96-well plates in NSC medium (n⩾6). Numbers of wells that had developed neurospheres 2 weeks postseeding were plotted versus the numbers of cells seeded. (C) wt as well as bulk and cloned eGFP/bcl2 neurosphere cells produced under differentiation conditions GFAP⁺ (glial fibrilaric acidic protein) astroglial cells (top) and tubulin beta-III⁺ neuronal cells (bottom). Cells were subjected to nuclear counterstaining (DAPI).