Table 1.

RT-PCR analysis of clc, clf and cntfr expression^a

	clc		clf		cntfr
	E16.5	E18.5	E16.5	E18.5	E16.5	E18.5
Skeletal muscle	0.113 ± 0.02	0.936 ± 0.12	0.232 ± 0.003	24.84 ± 2.13	90.25 ± 2.71	6.72 ± 0.76
Heart	NSc	NS	NS	NS	NS	NS
Tongue	3.12 ± 0.12	1.2 ± 0.09	495 ± 17.1	77.1 ± 4.64	28.3 ± 10.9	2.6 ± 0.83
Muzzle	11.4 ± 0.75	9.25 ± 0.79	1050 ± 65.3	524 ± 85.8	425 ± 47.5	57.9 ± 4.02
Lung	4.08 ± 0.65	16.4 ± 0.48	2290 ± 490	2240 ± 184	43.8 ± 15.6	NDb
Kidney	5.55 ± 0.12	6.75 ± 1.15	61.4 ± 4.59	ND	51.8 ± 9.49	19.8 ± 3.12
Liver	0.191 ± 0.02	0.129 ± 0.03	0.071 ± 0.001	0.034 ± 0.09	0.047 ± 0.002	0.038 ± 0.03
Brain	1.04 ± 0.12	0.317 ± 0.09	150 ± 3.39	74.3 ± 16.4	216 ± 2.01	236 ± 16.1
Spinal cord	ND	0.183 ± 0.01	0.123 ± 0.01	61.2 ± 6.25	6.13 ± 0.03	109 ± 27.4

^aExpression of clc, clf and cntfr was determined using reverse transcription and quantitative real-time PCR as detailed in Experimental Procedures and expressed as fM of cDNA/μg total RNA.

^bnot determined

^cnot significant