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. 2017 May 23;69(7):463–478. doi: 10.1007/s00251-017-0993-7

Fig. 3.

Fig. 3

Alignment of 47 Icelandic black-tailed godwit MHC-I alleles covering 244–247 bp of the α2 domain (exon 3) sequenced with Illumina MiSeq. Identity to Lili-UA*03 is indicated by dots, and dashes indicate true gaps. Alleles Lili-UA*01, 02, 05, 08, 09, 14, 15, 17, and 40 have a 3 bp deletion at position 147. Alleles Lili-UA*04, 28, 30, 47 encode for the same amino acid, as does Lili-UA*7, 46; Lili-UA*11, 12; Lili-UA*22, 42, and Lili-UA*35, 43. The boxes indicate the peptide-binding residues (PBR) inferred from Wallny et al. (2006), excluding peptide main-chain sites. Residues under positive selection (+) and negative selection (N) were calculated using the SLAC, FEL, and REL methods (www.datamonkey.org; Pond et al. 2006; Delport et al. 2010)