Table 1.

Identification of obtained isolates and growth observations with SLES, under nitrate-reducing conditions

Strain code	Identification	Growtha	Enrichmentb
S1	Aeromonas hydrophila (99% similar to A. hydrophila DSM 30187T)	+/−	50
S3	A. hydrophila (99% similar to A. hydrophila DSM 30187T)	+/−	250
S6	Pseudomonas nitroreducens (99% similar to P. nitroreducens DSM 14399T)	+/−	250
S7	A. hydrophila (99% similar to A. hydrophila DSM 30187T)	+/−	250
S8	Pseudomonas stutzeri (99% similar to P. stutzeri CCUG 11256T)	+	1000
S10	P. stutzeri (99% similar to P. stutzeri CCUG 11256T)	+	1000
S11	P. nitroreducens (99% similar to P. nitroreducens DSM 14399T)	+	1000

Based on 16S rRNA gene comparison (http://ncbi.nlm.nih.gov/blast), strains S1, S3, and S7 are 99% similar; strains S6 and S11 are 99% similar; strains S8 and S10 are 99% similar. All strains were also tested for SLES degradation with oxygen as electron acceptor; similar growth observations were obtained as presented in this table

^aGrowth observations were compared to controls without SLES; +/− weak but visible growth; + growth

^bOriginal enrichment where the strain was isolated from; 50, 250, and 1000 correspond to the 50, 250, and 1000 mg SLES L⁻¹ enrichments, respectively