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Quantitation of IbpA DR2 expression in diatoms by Western analysis. Wt wild-type diatoms, DR2-1 and DR2-2 batches 1 and 2 of IbpA DR2 expressing diatoms, DR2 H:A-1 and DR2 H:A-2 batches 1 and 2 of diatoms expressing IbpA DR2 with the critical histidine in the catalytic site replaced by alanine. Quantitation was based on densitometric comparisons with known amounts of DR2 protein
