Fig. 3.

Human adult cardiac myocytes are protected from H₂O₂-induced apoptosis by uPA and ATF. Human adult cardiac myocytes (HACM) were incubated with 200 µM H₂O₂ or left untreated for 24 h (A). HACM were treated with 100 U/ml uPA (B) or 50 ng/ml ATF (C) or left untreated for 24 h before addition of 200 µM H₂O₂ for 24 h. Apoptosis was quantified as described in “Materials and methods”, apoptotic cell nuclei appear as green. Values are given as x-fold apoptotic cells over respective control and represent mean of 3 determinations ± SD of five representative microscopy fields each. The inset to B shows representative TUNEL staining images of the different conditions, the white bar represents 60 µm