Fig. 5.

Phylogenetic tree of chloroplast genotypes obtained from the saline soda lime samples based on the pyrosequencing of the 16S rRNA gene. Tree was constructed using the Maximum Likelihood method with the General Time Reversible nucleotide substitution model and is based on 334 nucleotide positions. GenBank accession numbers are given in parentheses. Representative sequences obtained by pyrosequencing are marked with grey squares. In this case, sequences differing only in one position are represented with a single sequence, number of sequences is given in square brackets (I interior and S surface sample) and major phylotypes representing at least 0.5% of total chloroplast sequences are highlighted with bold letters