Fig. 5.

Overexpression of Stim1 decreases sensitivity of osteosarcoma cells to cisplatin treatment. a MG63 were transfected with Stim1 plasmid using Lipofectamine Plus reagent (Lipo) for 24 h. Stim1 expression was examined by western blotting. **P < 0.01 versus control, n = 4. b Quantification of the thapsigargin (Tg)-induced SOCE amplitude in MG63 cells transfected with Stim1 plasmid in the presence of cisplatin (10 μM). **P < 0.01 versus control; ^# P < 0.05, ^## P < 0.01 versus cisplatin alone, n = 6. c MG63 cells were transfected with Stim1 plasmid for 24 h and then were incubated with cisplatin for another 24 h. Cell viability was determined using CCK-8 assay. **P < 0.01 versus Lipo; ^## P < 0.01 versus Lipo + cisplatin, n = 6. d Cell apoptosis was analyzed by Annexin V-FITC/PI staining followed by flow cytometry. e After treatments mentioned in c, the protein expressions of GRP78, CHOP, ATF4 and Stim1 were determined by western blotting. f Densitometric analysis of the above protein expressions. **P < 0.01 versus Lipo; ^## P < 0.01 versus Lipo + cisplatin; ^&& P < 0.01 versus Stim1, n = 6