Fig. 2.

TRAP synthesis. 17% (w/v) SDS-PAGE analysis of the whole cell lysate samples from overexpressed cell cultures. Proteins were stained with Coomassie Brilliant Blue. Lane 1 bacterial sediment after 9 h culture with IPTG at 37 °C; lane 2 bacterial sediment after 16-h culture with IPTG at 37 °C; lane 3 bacterial sediment from non-recombinant bacteria after 16-h culture with IPTG at 37 °C, negative control; lane 4 bacterial sediment after 9-h culture with IPTG at 4 °C; lane 5 bacterial sediment after 16-h culture with IPTG at 4 °C; lane 6 bacterial sediment from non-recombinant bacteria after 16-h culture with IPTG at 4 °C, negative control; lane 7 molecular mass markers (SigmaMarker low molecular weight range 6.5–66 kDa); lane 8 culture medium after 16-h culture with IPTG at 37 °C. Arrows indicate recombinant peptide