Table 2.
EMP added, µg/mL | Cell index value, mean ± standard deviation | ||||
---|---|---|---|---|---|
Time of incubation | |||||
12-h | 24-h | 48-h | 60-h | 72-h | |
Control | 0.26 ± 0.17 | 0.30 ± 0.20 | 0.39 ± 0.26 | 0.56 ± 0.38 | 0.86 ± 0.60 |
EMD, 12.5 | 0.29 ± 0.10 | 0.35 ± 0.10 | 0.45 ± 0.13 | 0.61 ± 0.18 | 0.88 ± 0.32 |
EMD, 25.0 | 0.17 ± 0.11 | 0.19 ± 0.13 | 0.24 ± 0.17 | 0.35 ± 0.27 | 0.54 ± 0.45 |
EMD, 50.0 | 0.26 ± 0.16 | 0.31 ± 0.19 | 0.38 ± 0.23 | 0.47 ± 0.30 | 0.62 ± 0.43 |
prAMEL, 12.5 | 0.28 ± 0.04 | 0.31 ± 0.02 | 0.48 ± 0.03 | 0.90 ± 0.06 | 1.59 ± 0.13 |
prAMEL, 25.0 | 0.35 ± 0.05 | 0.39 ± 0.01 | 0.57 ± 0.05 | 1.24 ± 0.15 | 1.84 ± 0.26 |
prAMEL, 50.0 | 0.19 ± 0.08 | 0.28 ± 0.12 | 0.43 ± 0.05 | 1.01 ± 0.14 | 1.80 ± 0.22 |
TRAP, 12.5 | 0.42 ± 0.06 | 0.48 ± 0.06 | 0.66 ± 0.06 | 1.07 ± 0.15 | 1.69 ± 0.16 |
TRAP, 25.0 | 0.37 ± 0.06 | 0.41 ± 0.06 | 0.51 ± 0.05 | 0.75 ± 0.06 | 1.09 ± 0.16 |
TRAP, 50.0 | 0.48 ± 0.06 | 0.54 ± 0.06 | 0.68 ± 0.06 | 0.95 ± 0.05 | 1.33 ± 0.10 |
P value | >0.05 | >0.05 | >0.05 | 0.0001 | 0.0001 |
Cell index values were monitoring using the xCELLigence system. Results are from five repeats
EMP enamel matrix protein, EMD enamel matrix derivative, prAMEL porcine recombinant amelogenin, TRAP tyrosine-rich amelogenin peptide