Table 1.
Primer sequences and amplification conditions for RT-PCR analysis
| Gene | Primer sequences, 5′ to 3′ | Product size (bp) | Annealing temp. ( °C) | PCR cycles | GenBank accession number |
|---|---|---|---|---|---|
| Vimentin | F: GGGACCTCTACGAGGAGGAG | 200 | 55 | 35 | NM_003380 |
| R: CGCATTGTCAACATCCTGTC | |||||
| Collagen I | F: CCAAATCTGTCTCCCCAGAA | 214 | 55 | 35 | NM_000088 |
| R: TCAAAAACGAAGGGGAGATG | |||||
| Runx2 | F: CCCCACGACAACCGCACCAT | 292 | 59 | 35 | NM_001015051 |
| R: GTCCACTCCGGCCCACAAATC | |||||
| Nestin | F: AACAGCGACGGAGGTCTCTA | 220 | 55 | 35 | NM_006617 |
| R: TTCTCTTGTCCCGCAGACTT | |||||
| Nanog | F: ACCTTCCAATGTGGAGCAAC | 199 | 55 | 35 | NM_024865 |
| R: GAATTTGGCTGGAACTGCAT | |||||
| Oct3/4 | F: GACAGGGGGAGGGGAGGAGCTAGG | 144 | 60 | 35 | NM_001173531 |
| R: CTTCCCTCCAACCAGTTGCCCCAAAC | |||||
| Sox2 | F: AACCCCAAGATGCACAACTC | 152 | 60 | 40 | NM_003106 |
| R: CGGGGCCGGTATTTATAATC | |||||
| GAPDH | F: GAGTCAACGGATTTGGTCGT | 238 | 55 | 35 | NM_002046 |
| R: TTGATTTTGGAGGGATCTCG | |||||
Collagen I Collagen type I alpha 1 chain, Runx2 Runt-related transcription factor 2, Nanog Nanog homeobox, Oct3/4 POU class 5 homeobox 1 (POU5F1), Sox2 SRY-box 2, GAPDH glyceraldehyde-3-phosphate dehydrogenase