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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2016 Sep 24;101:32–43. doi: 10.1016/j.freeradbiomed.2016.09.019

Figure 3. Effects of antioxidant proteins SOD1 and catalase on the expression of PAI-1 gene and the affinity of p300 association to DNA.

Figure 3

(A) MnTE-2-PyP reduces PAI-1 gene expression with 20 Gy and 5 Gy of radiation. The gene expression levels of PAI-1 was measured by real-time PCR using the ΔΔCT method. 18S was used as the housekeeping gene. (B) PAI-1 mRNA expression in PC3 cells overexpressing SOD1 or catalase. Cells were exposed to 20 Gy of radiation. 24 hours later, RNA was isolated from PC3 cells and relative levels of human PAI-1 mRNA were analyzed by quantitative RT-PCR using the ΔΔCT method. 18S was used as the housekeeping gene. (C) Affinity for p300 to the HRE binding site on PAI-1 gene promoter region changes after SOD1 and catalase transfection. (D) MnTE-2-PyP inhibits p300 binding to the PAI-1 promoter in a dose dependent manner. Data represent mean ± SD from 3 independent experiments. * p < 0.05, ** p < 0.01 compared to control groups, as analyzed with ANOVA followed by two-tailed Student’s t-test.