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. 2017 Jun 27;12(6):e0179740. doi: 10.1371/journal.pone.0179740

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© 2017 Pröschel et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 11 — (A) Sequence alignment of different 3kptC^T-MBP variants (I: 3kptC^T wildtype (residues T505-K518), II: N-terminal N506-Q507 extension of 3kptC^T, III: C-terminal GWI instead of PTK in 3kptC^T). (B) Comparative covalent intermolecular bond formation assay between different 3kptC^T-MBP variants and mCherry-3kptC^C. Purified 3kptC^T variants and mCherry-3kptC^C proteins were mixed each at 15 μM (final conc.) for 24h at 25°C with shaking at 500 rpm before boiling (10min, 95°C) and SDS-PAGE with Coomassie staining. Interaction I: mCherry-3kptC^C + 3kptC^T (wildtype), interaction II: mCherry-3kptC^C + 3kptC^T (NQ), interaction III: mCherry-3kptC^C + 3kptC^T (GWI). (lane 1: mCherry-catcher input (30μM), lane 2: tag input (30μM), lane 3: 0h, lane 4: 1h, lane 5: 2h, lane 6: 3h, lane 7: 4h, lane 8: 24h). Same volume of samples were loaded. MW stands for molecular weight (kDa).