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. 2017 Jun 15;13(6):e1006404. doi: 10.1371/journal.ppat.1006404

Fig 10. Differentiation of epithelial cells induces accumulation of HIF-1α.

Fig 10

Immunoblots showing changes with time in HIF-1α protein levels following induction of differentiation of (A) NOK and (B) NOK-Akata cells by suspension in methylcellulose (1.6% in K-SFM) for the times indicated. (C) Immunoblots showing HIF-1α accumulation in hGET cells following induction of differentiation by suspension in methyl cellulose (1.6% in K-SFM for 48 h; lane 2). As controls, the cells for lanes 1, 3, and 4 were incubated in parallel in K-SFM without MC. For lane 4, 50 μM DFO was added to the cells 24 h prior to harvest as a control for HIF-1α stabilization in the absence of MC-induced differentiation. (D) Immunoblots showing HIF-1α accumulation in NOK (clone #3) cells following induction of differentiation by growth in organotypic culture for 11 days at an air-liquid interface. Lanes 2–4 contain protein extracts from three different rafts. Lane 1 contains protein extract from NOK (clone #1) cells maintained in an undifferentiated state as a monolayer grown in K-SFM. Whole-cell extracts were prepared and analyzed for the indicated proteins.