Figure 9. Affimer selection and specificity against TNT and DNT’s.
(A) Chemical structures of TNBS, TNT, 2,3-DNT, 2,4-DNT and 2,6-DNT. (B) Phage ELISA results from 32 monoclonal Affimer reagents isolated against TNBS bound to ovalbumin. Binding specificity was also tested against TNBS bound to IgG and to unconjugated ovalbumin and IgG. (C) Competition ELISA of four TNT-Affimers to check specificity against a range of molecules across a concentration profile. Error bars = standard deviation from technical repeats of a representative ELISA.