(
A) Immunoblots for PABPC1 from postnatal day 0 (P0) and 8-week-old adult mice from the indicated tissue with GAPDH as a loading control. (
B) Quantification of PABPC1 protein and mRNA expression (qPCR). Data are mean ± s.d (n = 3); *p<0.005 unpaired two-tailed
t-test. (
C) PABPC1 immuno-histochemistry images based on anti-PABPC1 antibody (Abcam ab21060) from the Human Protein Atlas Database (
www.proteinatlas.org) showing that PABPC1 is abundantly expressed in other adult tissues but not heart and skeletal muscle. (
D) RPKM values from the Human Protein Atlas Database showing relative
Pabpc1 mRNA levels in various human tissues. (
E) Western blot of purified P0 and adult cardiomyocytes (CMs) and cardiac fibroblasts (CFs). Blotting for Desmin (DES, CM marker) and Vimentin (VIM, CF marker) show clean separation of cell types.