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. 2017 Jun 27;6:e25306. doi: 10.7554/eLife.25306

Figure 1. Cellular I-BET151 dose response curves in MLL-fusion leukaemia and non-MLL-fusion leukaemia cell lines.

Cell viability assays were performed for a MLL-fusion leukaemia cell line (MV4;11) and a non-MLL-fusion leukaemia cell line (K-562) with the I-BET151 inhibitor. Curves and absolute IC50 values were determined for each biological repeat. (A) Dose response curves for each biological repeat [n = 5] for the MV4;11 cell line. Percent cell viability is relative to DMSO treated cells. I-BET151 doses range from 1 pM to 1 mM. Exploratory one-sample t-test of IC50 values compared to a constant of 100 µM; t(4) = 20.63, p=3.26×10−5; Cohen’s d = 9.23, 95% CI [3.13, 15.45]. (B) The mean absolute IC50 value for MV4;11 cells and 95% confidence interval [n = 5] for this replication attempt is plotted with the IC50 value reported in Dawson et al. (2011) displayed as a single point (red circle) for comparison. (C) Dose response curves for each biological repeat [n = 4] for the K-562 cell line. Percent cell viability is relative to DMSO treated cells with I-BET151 dose range from 1 pM to 1 mM. The IC50 estimates were not able to be accurately determined following published guidelines (Sebaugh, 2011). Additional details for this experiment can be found at https://osf.io/zm3j4/.

DOI: http://dx.doi.org/10.7554/eLife.25306.002

Figure 1.

Figure 1—figure supplement 1. Cellular I-BET151 dose response curves for each MV4;11 biological repeat.

Figure 1—figure supplement 1.

This is the same experiment as in Figure 1. The dose response curve of each biological repeat for the MV4;11 cell line plotted individually with the absolute IC50 value listed. Additional details for this experiment can be found at https://osf.io/zm3j4/.
DOI: http://dx.doi.org/10.7554/eLife.25306.003
Figure 1—figure supplement 2. Cellular I-BET151 dose response curves for each K-562 biological repeat.

Figure 1—figure supplement 2.

This is the same experiment as in Figure 1. The dose response curve of each biological repeat for the K-562 cell line plotted individually. The IC50 estimates were not able to be accurately determined following published guidelines (Sebaugh, 2011), and are listed as greater than 100 µM, which is the highest dose before inhibition started to be observed. Additional details for this experiment can be found at https://osf.io/zm3j4/.
DOI: http://dx.doi.org/10.7554/eLife.25306.004