Figure 2. BCL2 expression in I-BET151 treated MV4;11 and K-562 cells.
MV4;11 and K-562 cells were treated with 500 nM I-BET151, or an equivalent volume of DMSO. Total RNA was isolated 6 hr after treatment and qRT-PCR was used to detect BCL2 and B2M expression. Fold change in BCL2 expression normalized to B2M and relative to DMSO is presented for I-BET151 treated MV4;11 and K-562 cells. Expression level of BCL2 in DMSO was assigned a value of 1. Means reported and error bars represent SD from three independent biological repeats. Two-sample t-test comparing fold gene expression values from MV4;11 cells to K-562 cells; t(4) = 17.23, uncorrected p=6.66×10−5, a priori Bonferroni adjusted significance threshold = 0.0167; (Bonferroni corrected p=0.0004). One-sample t-test comparing fold gene expression from K-562 cells to a constant of 1 (DMSO treated cells); t(2) = 3.53, uncorrected p=0.0719, a priori Bonferroni adjusted significance threshold = 0.0167; (Bonferroni corrected p=0.216). One-sample t-test comparing fold gene expression from MV4;11 cells to a constant of 1 (DMSO treated cells); t(2) = 17.86, uncorrected p=0.003, a priori Bonferroni adjusted significance threshold = 0.0167; (Bonferroni corrected p=0.009). Additional details for this experiment can be found at https://osf.io/np6gq/.