Figure 6.

Cx43 and Cx46 expression analysis in the GBM and the respective serum-free cell lines (GSCs) by WB (A) and immunofluorescence (B). (A) The expression of Cx43 and Cx46 in the GBM02, GBM11, OB1, and U87 cell lines and the respective serum-free cells, GBM02-SF, GBM11-SF, OB1-SF, and U87-SF, as previously described, was quantified by WB. Statistical analysis was performed in GraphPad Prism 5 for Windows (version 5.00; GraphPad Software, Inc., San Diego, CA). Each value represents the mean ± SEM from three independent experiments; *P < .05, **P < .01. (B) Immunofluorescence staining of Cx43 and Cx46 was also performed in all cell lines. Cells were imaged at 63× magnification using a DMi8 advanced fluorescence microscope (Leica Microsystems, Germany) and analyzed with Leica LA SAF Lite. Images were processed using the software ImageJ 1.49v (Wayne Rasband, National Institutes of Health).