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. 2017 Jun 24;10(4):555–569. doi: 10.1016/j.tranon.2017.04.005

Figure 7.

Figure 7

Capability of GBM cell lines to form tumors in vivo. (A) Schematic depiction of GBM11 cell injection in striatum region of immunocompetent Swiss mouse brain. (B) MRI 14 days after GBM11 cell transplantation into a representative mouse brain. Coronal, sagittal, and axial cuts of tumor site in control and GBM11-injected mice. The asterisk (*) indicates the tumor mass. (C) Histopathological characteristics of the tumor mass in mouse brain parenchyma 14 days after implantation of GBM11 cells, revealed by hematoxylin-eosin staining. I: Neoplastic cells forming a circumscribed solid tumor mass in the brain tissue (black asterisk). II: Glomeruloid vessels (black asterisk). Microscopic analysis showed anaplastic cells and tumoral necrosis. (D) Immunohistochemical characteristics of the tumor revealed the expression of human vimentin (hVim). hVim staining (green) at the core of the tumor mass (red asterisk) and SOX2 (red), depicted by cell nuclei atypia (DAPI counterstaining in cyan blue, inset), and at the border of the tumor mass. Cells were imaged at 63× magnification using a DMi8 advanced fluorescence microscope (Leica Microsystems, Germany) and analyzed with Leica LA SAF Lite. Images were processed using the software ImageJ 1.49v (Wayne Rasband, National Institutes of Health).