Supplementary Figure 2.

Knockdown BIRC5. BIRC5 siRNA resulted in a partial decrease of cell viability (402-91, 69%; 1765-92, 55%; and DL-221, 69%). The transfection was successful as all three MLS cell lines and the control siRNA GAPDH showed a minor reduction of cell viability, whereas transfection with the positive control siPLK1 resulted in all three cell lines with a pronounced decrease in cell viability. The transfection reagent alone hardly affected cell viability (A). A reduction in GAPDH and survivin protein expression was found after transfection with, respectively, siGAPDH and siBIRC5 in all three cell lines, although knockdown efficiency was variable (B).