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. 2017 Jun 24;19(8):595–605. doi: 10.1016/j.neo.2017.04.006

Figure 7.

Figure 7

CD8-mediated cytotoxic mediators inhibit polyp growth in Stat6-deficient ApcMin/+ mice. (A), IFNγ expression by splenocytes from ApcMin/+ (n = 6), ApcMin/+ Stat6+/− mice (n = 8), and ApcMin/+ Stat6−/− mice (n = 7) that were stimulated in vitro with 0.5 μg/ml of CD3 and CD28 antibodies. (B) mRNA expression of granzyme B and perforin in polyps and nontumor small intestine tissue of ApcMin/+ (n = 5), ApcMin/+ Stat6+/− mice (n = 4), and ApcMin/+ Stat6−/− mice (n = 5). Statistical significance was determined by two-way analysis of variance. (C) TUNEL assay showing apoptosis of polyp epithelial cells in ApcMin/+ (n = 3) and ApcMin/+ Stat6−/− mice (n = 3). TUNEL-positive cells were counted in three to four fields per polyp/mouse. * P < .05, ** P < .005, *** P < .0005, **** P < .0001.