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. 2017 Jun 27;7:4308. doi: 10.1038/s41598-017-04661-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ach and SNP-mediated vasorelaxation in KCl- precontracted thoracic aortas isolated from DHI-treated SHR. (A,B) Raw trace of Ach and SNP-treated aorta and (C,D) Quantification of the data in (A and B) respectively. The rate of Ach and SNP-induced relaxation was calculated with KCl (60 mM)-induced contraction set to 0. (C) DHI treatment significantly (p < 0.01) relaxed aorta in response to Ach stimulation compared with control group in SHR. (D) There was no significant difference in response to SNP stimulation between DHI treatment group and control group in SHRs’ aorta. All data are expressed as mean ± SEM, n = 5.