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. 2017 Jun 27;7:4269. doi: 10.1038/s41598-017-04105-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genome wide organization of grape TLP (VvTLP) genes. (a) Phylogenetic tree based on the protein sequences of 33 VvTLP genes. Phylogenetic tree was constructed using the neighbor-joining method with MEGA5. Four subfamilies (Type I, II, III and IV) were analyzed and colored in red, yellow, blue and green, respectively. Bootstrap values at the nodes from 1000 replicates were used to assess the robustness of the tree. The scale is in amino acid substitutions per site. (b) Exon-intron structure of VvTLP genes: yellow indicates coding sequence (CDS), blue indicates untranslated 5′- and 3′- regions, black indicates introns. (c) Structures of VvTLP proteins: brown indicates Thaumatin_2 domain, blue rectangle indicates Thaumatin_1 domain, green ellipse in VvTLP21 and VvTLP23 indicates a PROKAR-lipoprotein domain, green ellipse in VvTLP18 indicates a FE2OG_OXY domain, blue pentagon indicates an ARM_REPEAT domain, blue ellipse indicates a HEAT_REPEAT domain, gray line indicates a disulfide bridge, gray icon indicates active sites, green line indicates undefined bridge/range.