Figure 4.

Response of VqTLP29 over-expressing Arabidopsis thaliana lines to powdery mildew inoculation. (a) Phenotypes of the 4-week old VqTLP29 transgenic line L1, L2, L3 and Col-0 infected with powdery mildew and disease symptoms in representative rosettes after inoculation for 7 days. (b) The number of spores per gram fresh leaf 7 days post powdery mildew inoculation. Spore suspensions of the VqTLP29 transgenic lines and Col-0 were diluted in sterile water with 10 diseased leaves and counted using the hemocytometer. Data were combined and analyzed as a one-way ANOVA in three independent experiments. Asterisks indicate statistical significance (**P < 0.01). (c) Callose deposition, cell death and O²⁻ accumulation in the VqTLP29 transgenic lines and Col-0 7 days post powdery mildew inoculation. Three infected leaves of the transgenic lines and Col-0 were required for each stain in three independent experiments. The scale bar in the figure showing aniline blue staining indicates 100 μm. (d) Expression levels of disease resistance genes in the VqTLP29 transgenic lines and Col-0 at 0, 24, 72 and 120 hpi following powdery mildew inoculation. Asterisks indicate statistical significance (*0.01 < P < 0.05, **P < 0.01, one-way ANOVA). The experiments were repeated three times with consistent results.