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. 2017 Jun 27;7:4289. doi: 10.1038/s41598-017-03598-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Multiplexing the platform to acquire quantitative growth and fluorescent data. Different conidial concentrations of the H1-RFP (a) and the gamma-actin-GFP (b) strains were inoculated on solid media containing ammonium as the sole nitrogen source. Growth was monitored by measuring absorbance at 595 nm (grey and black symbols). In parallel, fluorescence was detected as described in the Methods section (red symbols: fluorescence in the red channel; green symbols: fluorescence in the green channel).