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. 2017 Jun 27;7:4289. doi: 10.1038/s41598-017-03598-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hourly monitoring of the expression of the secondary metabolite genes during the entire fungal life cycle. Monitoring expression of aflR. (a) A. nidulans aflR(p)::gfp was grown on solid minimal media with ammonium (repressing) or nitrate (inducing) as sole nitrogen source. Media was supplemented with 0 µM or 1 µM trichostatin A (TSA), a histone deacetylase inhibitor known to increase SM gene expression of some SM gene clusters. (b) A. nidulans aflR(p)::gfp in a wild type (WT) or ΔkdmB mutant background were grown on solid minimal media with ammonium as sole nitrogen source at 37°C. Fluorescence and hence aflR expression is decreased upon deletion of kdmB. Addition of TSA increased the expression of aflR in both the wild type and the ΔkdmB mutant, as shown by an increase in the fluorescence readings. Growth and GFP fluorescence were monitored in parallel. Hyphal growth was only slightly affected upon deletion of kdmB as well as TSA treatment, as shown in the corresponding growth curves in Supplementary Fig. 4. The diagrams depict representative examples of the fluorescence patterns as the average from 3–4 samples.