Figure 6.

Monitoring expression of TRI5(p)::gfp and CPS/KS(p)::gfp in F. graminearum and F. fujikuroi. (a) The F. graminearum wild-type strain was grown on solid Fusarium minimal medium (FMM) with either 5 mM L-ornithine (ORN, inducing conditions) or 50 mM nitrate (NO₃⁻, repressing conditions) at 24°C up to 72 h. Absorbance at 595 nm was monitored every hour. (b) In parallel, TRI5 expression was quantified with the levels of GFP fluorescence. It was clearly detectable under inducing conditions, while fluorescence was not significantly detectable under repressing conditions. (c) The F. fujikuroi wild-type strain was grown on solid ICI medium with either 6 mM glutamine (GLN, inducing) or 60 mM glutamine (repressing) at 28°C up to 66 h. Fluorescence and hence CPS/KS expression is increased under inducing conditions compared to repressing conditions. (d) Addition of TSA to the culture media reduced the expression of CPS/KS grown under inducing conditions. Hyphal growth did not differ markedly between the different conditions (Supplementary Fig. 5). In all cases, data were acquired every one hour over the complete time course. The diagrams depict representative examples of the fluorescence patterns as the average from 3–4 samples.