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. 2017 Jun 27;7:4263. doi: 10.1038/s41598-017-02800-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-15a/16 expression is altered in chemically induced DNA damage and it suppresses the BMI1 expression by targeting 3′UTR. Heat map showing the endogenous expression of miRNAs. The Green colour in the heat map indicates decreased and red colour indicates increased mRNA expression level. U6 was used as endogenous control (A). Predicted miRNA sequences and their recognition sites within 3′UTR of BMI1 (B) Renilla luciferase reporter assay showing the reporter expression in MCF-7 and MDAMB-231 cells co-transfected with wild-type 3′UTR of BMI1and mutant 3′UTR of BMI1 along with miR-15a, miR-16. Scramble miRNA vector used as a control. Renilla luciferase activity was normalized to firefly luciferase. n = 3, Students t-test was used to generate P values, *, ** and*** indicates P value summary (C,D). Western blot and RT-PCR data showing the expression of BMI1 upon overexpressed miR-15a, miR-16 and both. Blots were cropped to enhance the representation (E).