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. 2017 Jun 27;7:4263. doi: 10.1038/s41598-017-02800-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-15a/16 sensitizes the breast cancer cells to the chemotherapeutic drug Protein expression of BMI1, Ub-H2A, BID, BCL2, Caspase-3, p-AKT, GSK3β were checked in MDAMB-231 cells ectopically expressed with miR-15a, miR-16 or both miR-15a/16 and treated with doxorubicin. Actin served as gel loading control. Scramble miRNA vector was used as transfection control. Blots were cropped to enhance the representation (A). Results of MTT assay showing the effect of miR-15a, miR-16 treated with doxorubicin in MDAMB-231 cells. Scramble miRNA vector was used as control. Error bars indicate ± S.E. of n = 3 (B). Tunel assay showing the apoptotic cascade of miR-15a, miR-16 ectopically expressed in MDAMB-231 cells treated with doxorubicin. Scramble miRNA vector was used as control. Bar indicates 200 μm (C). Acridine orange/Ethidium bromide double staining represents the live (Green), early apoptotic (Yellow) and late apoptotic/dead (orange/red) cells of miR-15a, miR-16 ectopically expressed in MDAMB-231 cells treated with doxorubicin. Bar indicates 100 μm (D).