Figure 4.

Effects of ETH129 on isolated mouse cardiomyocytes. (a and b) Isolated mouse adult cardiomyocytes were co-loaded with calcein, CoCl₂ and TMRM at 37 °C and then incubated in a Tyrode’s buffer. After 5 min incubation ETH129 (arrow) was added to the medium. (c) ETH129 induces a concentration-dependent hypercontracture of cardiomyocytes. (d) Isolated mouse adult cardiomyocytes were co-loaded with calcein, CoCl₂ and propidium iodide (PI) at 37 °C and then incubated in a Tyrode’s buffer. After 5 min incubation ETH129 (20 µM; arrow) was added to the medium. (e and f) Cardiomyocytes isolated from wild type (WT) or CypD knock out (KO) mice were co-loaded with calcein, CoCl₂ and TMRM at 37 °C and then incubated in Tyrode’s buffer. After 5 min incubation, 20 µM ETH129 (arrow) was added to the medium. Fluorescence values were obtained by averaging pixel intensities after background substraction and were normalised as a percentage of the initial value. Each trace represents the mean ± SEM of 3–5 experiments.