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. 2017 Jun 27;7:4271. doi: 10.1038/s41598-017-04133-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of GATA2 or GATA3 knockdown on HSD3B1 expression. JEG-3 cells were transfected with shRNA specific for GATA2, GATA3, or LacZ (negative control). (A,C) Cell lysates were analysed by western blot analysis. (B,D) RNA was extracted and quantified by qRT-PCR. Values are means ± SEM of three independent experiments. **P < 0.01, ***P < 0.001 compared with the control LacZ (Student’s t-test). Uncropped images of blots were shown in Supplementary Fig. S6.