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. 2017 Jun 27;7:4265. doi: 10.1038/s41598-017-04069-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibitory effect of serum derived from A. hydrophila DNA stimulated zebrafish on the growth of A. hydrophila. (A) Growth curve of A. hydrophila (A.h) in M9 medium cultured in the presence of unstimulated serum (Unstimulated), A. hydrophila DNA (1 μg per fish) stimulated serum (Stimulated), heat-treated stimulated serum (Heated), stimulated serum neutralized with anti-hepcidin Ab (Hepcidin Ab), stimulated serum neutralized with anti-hepcidin Ab plus 10 μM Fe²⁺ supplemented ones (Hepcidin Ab + Fe²⁺), and A. hydrophila cultured alone in M9 (Mock). (B) Detection of the bacterial generation time of A. hydrophila cultured in the presence of different serum samples (unstimulated, stimulated, and Hepcidin Ab + Fe²⁺) in M9 medium for 8 h. (C) Detection of the absorbance (A₆₀₀) values of A. hydrophila cultured in the presence of different serum samples (unstimulated, stimulated, and Hepcidin Ab + Fe²⁺) in M9 medium at 8 h time point. All data are representative of at least three independent experiments. *p < 0.05, **p < 0.01.