Table 2.

Influence of initial graphene protein loading on the catalytic properties of immobilized particle.

Enzyme loading (g/g of support)	Immobilization yield (%)	Immobilized protein loading (g/g of support)	Hydrolytic activity (U/g of biocatalyst)	Specific activity (U/gIP)
0.2	52.19 ± 1.32	0.10 ± 0.02	40.12 ± 1.23	401.05 ± 3.51
0.4	65.69 ± 2.36	0.26 ± 0.08	78.45 ± 3.28	301.56 ± 2.45
1	28.09 ± 0.10	0.28 ± 0.06	92.78 ± 2.11	331.15 ± 1.87
1.5	22.00 ± 1.35	0.33 ± 0.01	101.36 ± 3.82	307.02 ± 3.88
2	18.98 ± 0.12	0.37 ± 0.02	124.07 ± 1.76	335.14 ± 2.65
2.5	18.61 ± 0.56	0.47 ± 0.01	144.81 ± 2.26	308.18 ± 1.45
3	23.05 ± 2.83	0.69 ± 0.03	171.93 ± 2.55	249.15 ± 3.93
4	28.25 ± 1.28	1.13 ± 0.13	265.45 ± 1.12	234.93 ± 1.23
5	23.00 ± 0.21	1.15 ± 0.21	270.17 ± 2.64	234.89 ± 2.46

Reaction condition: Different concentrations of enzyme solution (1, 2, 5, 7.5, 10, 12.5, 15 and 20 g/L) was dissolved by 2 mL disodium hydrogen phosphate-citrate buffer (pH 7); and reaction temperature was 50 °C; the nanoparticles mass was 10 mg; the mixture was stirred at 120 rpm in an incubator shaker for 3 h.