Figure 29.
Multiscale super-resolution image data reveals critical contextual information. The plot displays a 3D multiresolution microscopy measurement of a particle approaching the membrane of a fibroblast cell. The fast diffusing nanoparticle was tracked using a 3D SPT module (the moving particle was kept in the focus of the objective via a piezoelectric stage and an optical feedback loop running at 100 kHz). The background (cell membrane, nucleus, etc.) was tracked with a conventional two-photon scanning fluorescence microscope. Valuable “contextual information” along single particle tracks is obtained from the combination of these two modalities. The arrows show trajectory segments where the nanoparticle traced out structured protrusions on the cell boundary.15 Reproduced with permission from ref 15. Copyright 2015, Royal Society of Chemistry.