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. Author manuscript; available in PMC: 2017 Jun 28.
Published in final edited form as: Chem Rev. 2017 Apr 17;117(11):7276–7330. doi: 10.1021/acs.chemrev.6b00729

Figure 3.

Figure 3

Microscope seen as a telescope. (a) A microscope’s resolution is determined by the numerical aperture NA of its objective, which is defined as the product of the index of refraction of the medium between the objective and the sample, n, and the sine of the half angular aperture, θ. (b) A telescope’s angular resolution is determined by its (physical) aperture, D. The intensity profile and diffraction spots were plotted using a simple Python script.