Figure 7.

Colocalization of PSD95 and PMCA4 in brain regions. Sections of control or ketamine treated brain regions: cortex (A), cerebellum (B), hippocampus (C) and striatum (D) were prepared as described in “Materials and Methods” Section and immunostained with anti-PMCA4 primary and respective Alexa Fluor 568 conjugated secondary antibody (shown in red) as well as with anti-PSD95 primary antibody and respective Alexa Fluor 488 conjugated secondary antibody (shown in green). Cell nuclei were counterstained with Hoechst33342 and are shown in blue. Representative and corresponding regions of interest are shown for each brain section. Colocalization percentage (fraction of PMCA4-positive pixels which are also positive for PSD95) was calculated for several regions of interest and its mean value ± SD is presented on the microphotographs. The examples of overlapping pixels are indicated by arrows. Scale bars: 10 μM. *P < 0.0001 ketamine treated vs. saline, n = 9.