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. 2017 Jun 28;11:172. doi: 10.3389/fncel.2017.00172

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Copyright © 2017 Xiong, Liu, Lu, Zhao, Dong, Liu, Zhang, Zhang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescent staining for BMSCs identification. (A) Immunofluorescent staining of CD45 in the cultured BMSCs, but no cells expressed CD45. (D,G) There were CD44 and CD29 red positive immunofluorescence (IF). (B,E,H) Cell nucleus was stained by DAPI with blue fluorescence. (C,F,I) The merged images showed the mean positive rate of CD44, CD29and CD45 were about 98.8%, 99.3% and 0%. Bar = 50 μm in (A–C), 25 μm in (D–I). The mean positive rate represented as mean number of the positive cells/the number of DAPI was analyzed using Image-Pro plus 6.0 software. CD44, CD 29, CD45 are the specific surface markers to identify the BMSC.