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. Author manuscript; available in PMC: 2017 Jun 28.
Published in final edited form as: Kidney Int. 2016 Mar;89(3):721–723. doi: 10.1016/j.kint.2015.10.019

Figure 1. C57BL/6N mice are susceptible to adriamycin-induced injury.

Figure 1

Adriamycin or saline was retro-orbitally injected in anesthetized C57BL/6N mice (n = 6–8) (15 mg/kg of body weight). Urine from each mouse was collected at 1-week intervals, and the 4-week urine samples were analyzed by sodium dodecylsulfate–polyacrylamide gel electrophoresis followed by Coomassie blue staining and enzyme-linked immunosorbent assay to calculate albumin-creatinine ratios. Albuminuria was noted only in adriamycin-injected mice (a and b; *P < 0.05). Scanning and transmission electron microscopy analysis of kidney tissues from these mice further confirms podocyte damage in adriamycin-injected mice only (c and d). BSA, bovine serum albumin.