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. 2017 Jun 28;8:741. doi: 10.3389/fimmu.2017.00741

Figure 3.

Figure 3

Cytokine production by human decidual NK (dNK) cells of the two groups. (A) mRNA levels and (B) protein levels of cytokines in decidual tissues of the two groups. (C) Representative purity analysis of sorted CD56+CD16 natural killer (NK) cells and CD56+CD94+ NK cells in induced abortion (IA) and recurrent spontaneous abortion (RSA). The purified CD56+CD94+ NK cells were subsequently used for real-time PCR and coculture. (D) mRNA levels of cytokines in fresh purified CD56+CD94+ dNK cells and (E) protein levels of cytokines in CD56+CD94+ dNK cultured 24 h after purification. (F) Representative density plots showing the cytokines in CD56+CD94+ dNK cells of IA and RSA with intracellular staining. (G) Percentage of cytokine expression in CD56+CD94+ dNK cells of IA and RSA, n = 6 for each group. The data are presented as the means ± SD. *P < 0.05 Student’s t-test.