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. 2017 Jun 28;8:741. doi: 10.3389/fimmu.2017.00741

Figure 4.

Figure 4

Decidual NK (dNK) cells of recurrent spontaneous abortion (RSA) reduce HTR-8/SVneo invasion and human umbilical vein endothelial cell (HUVEC) tube formation. (A,B) Transwell assay showed CD56+CD94+dNK cells culture supernatants of the two groups affects the number of invading HTR-8/SVneo cells. (A) Representative images of invading HTR-8/SVneo cells which were dyed with crystal violet. Magnification, 100×. (B) Average number of HTR-8/SVneo cells invading across the Matrigel toward dNK cells of different groups. (C,D) Formation by HUVECs of tube structures in vitro in conditioned supernatants obtained from CD56+CD94+ dNK cells of different groups. Values shown on each frame indicate average number of intersection. Magnification, 40×. The data are representative of three experiments. The data in panels (B,D) are presented as the means ± SD. **P < 0.01 versus control and induced abortion (IA) group. *P < 0.05 versus IA and RSA group. Student’s t-test.