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. 2017 Jun 28;8:741. doi: 10.3389/fimmu.2017.00741

Figure 5.

Figure 5

Decreased human leukocyte antigen (HLA)-G expression by miR-133a affected the function of decidual NK (dNK), including proinvasion and proangiogenesis. Overexpression of miR-133a reduced HLA-G expression in HTR-8/SVneo cells. (A) Relative miR-133a expression after transfection with mimics, inhibitor, and negative control (nc) in HTR-8/SVneo cells by qRT-PCR. (B,C) Relative HLA-G protein levels after transfection with miR-133a by western blot. The data are representative of three experiments. (D) Soluble HLA-G concentration in supernatant of HTR-8/SVneo cells transfected with miR-133a for 24 h. Cell culture supernatants were analyzed for (E) interleukin (IL)-8, (F) IP-10, (G) vascular endothelial growth factor (VEGF), (H) placental growth factor (PLGF), and (I) INF-γ.(J,K) Tube formation by human umbilical vein endothelial cells in vitro in conditioned supernatants obtained from CD56+CD94+ dNK cells cocultured with HTR-8/SVneo transfected with miR-133a negative control (nc), inhibitor, or mimics. Magnification, 40×. (L,M) Number of invading HTR-8/SVneo cells in conditioned supernatants obtained from CD56+CD94+ dNK cocultured with HTR-8/SVneo transfected with miR-133a nc, inhibitor, or mimics. Magnification, 100×. The data are representative of three experiments. The data are presented as the means ± SD. *P < 0.05. Student’s t-test.