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. 2017 Jun 28;8:741. doi: 10.3389/fimmu.2017.00741

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Copyright © 2017 Guo, Fang, Li, Xiao, Chen, Wang, Yang, Chen and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The impairment of proinvasion and proangiogenesis function of decidual NK (dNK) after blocking killer immunoglobulin-like receptor 2DL4 (KIR2DL4). (A) Cell culture supernatants were analyzed for IP-10, vascular endothelial growth factor (VEGF), placental growth factor (PLGF), INF-γ, and interleukin (IL)-8. The data are representative of three experiments. (B,C) Number of invading HTR-8/SVneo cells in conditioned supernatants obtained from CD56⁺CD94⁺ dNK cells cocultured with HTR-8/SVneo cells in the presence of anti-KIR2DL4 Ab or isotype. (D,E) Formation by human umbilical vein endothelial cells of tube structures in vitro in conditioned supernatants obtained from CD56⁺CD94⁺ dNK cells cocultured with HTR-8/SVneo in the presence of anti-KIR2DL4 antibody or isotype. The data in panels (C,E) are presented as the means ± SD. *P < 0.05. Student’s t-test.