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. 2017 Jun 28;8:1099. doi: 10.3389/fpls.2017.01099

FIGURE 4.

FIGURE 4

Effect of FaMRLK47 or FaMRLK50 overexpression (OE) and RNAi-mediated silencing of FaMRLK47 on strawberry (Fragaria × ananassa) fruit ripening. (A) RT-qPCR analysis of FaMRLK47 and FaMRLK50 expression in OE, RNAi, and control fruits. FaMRLK47 was overexpressed or silenced, and FaMRLK50 was overexpressed as described in Section “Materials and Methods.” The overexpression or RNAi constructs were injected into the fruits at 18 DPA and gene expression was analyzed 12 days after transfection with the OE vector or 8 days after transfection with the RNAi vector. Control samples were transfected with the empty vector (pCambia1304). RT-qPCR was conducted using FaACTIN as an internal control. Values are means ± SD of three biological replicates. Double asterisks denote significant difference at P < 0.01 using Student’s t-test. (B) The influence of FaMRLK47-OE, FaMRLK47-RNAi, and FaMRLK50-OE on the time course of strawberry fruit development and ripening. (C) The effect of FaMRLK47-OE and FaMRLK47-RNAi on the expression of ripening-related genes. Values are means ± SD of three biological replicates. Asterisks denote significant differences compared with the control sample (i.e., Empty vector) at ∗P < 0.05 and ∗∗P < 0.01, using Student’s t-test.